Reporter

Part:BBa_K4720105:Design

Designed by: Jelle de Boer   Group: iGEM23_Groningen   (2023-10-12)


Luciferase gene cassette


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 5274
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 2931
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1311
    Illegal SapI site found at 3450


Design Notes

The XbaI restriction site was removed to make the part RFC[10] compatible. Several RFC[1000] restriction sites remain.


Source

The luxCDABE operon was obtained from the pAKgfplux1 plasmid, which we ordered from AddGene.

References